02176nas a2200229   4500008004500000020001400045245011700059210006900176260000900245300001000254490000700264520135800271653002501629653002201654653002301676100002601699700001901725700001701744700002801761700002201789856013501811       2008                        Engldsh    a0370-432700aMolecular identification of Sitophilus spp. (Coleoptera Curculionidae) and detection of immature stages in wheat0 aMolecular identification of Sitophilus spp Coleoptera Curculioni  c2008  a55-580 v913 aA polymerase chain reaction (PCR)-based assay is proposed to differentiate Sitophilus granarius (L.), S. oryzae (L.), and S. zeamais Motschulsky at different life stages and to detect their hidden infestation in wheat kernels. PCR amplification of genomic DNA using primers designed on the basis of the cytochrome oxidase subunit II gene sequence, followed by agarose gel electrophoresis, Successfully detected the immature stages (egg, larva, pupa) and adult of each species. DNA amplification resulted in a PCR product of 462 bp for S. granarius, whereas S. oryzae and S. zeamais yielded a 311 bp fragment. A sequence comparison between the latter species allowed to identify a mutation that was diagnostic between S. oryzae and S. zeamais through a PCR-RFLP protocol. Digestion of DNA resulted in an uncut fragment (3 11 bp) for S. oryzae, two fragments (174 bp + 137 bp) for S. zeamais and 3 bands (250 bp + 160 bp + 52 bp) for S. granarius. Multiplex PCR reaction enabled to differentiate the three species at all life stages also when simultaneously added to and ground with a 50 g sample of wheat kernels. The reproducibility of the results and high resolution as well as the technical simplicity of the developed molecular method demonstrated its usefulness in the diagnosis of Sitophilus spp. and detection of their hidden infestation in wheat.10aSitophilus granarius10aSitophilus oryzae10aSitophilus zeamais1 aAngiolillo, Antonella1 aBoursier, Luca1 aPilla, Fabio1 aGerminara, Giacinto, S.1 aRotundo, Giuseppe  uhttps://weevil.myspecies.info/molecular-identification-sitophilus-spp-coleoptera-curculionidae-and-detection-immature-stages-wheat02549nas a2200205   4500008004100000020001400041245015000055210006900205260000900274300001000283490000600293520177900299653002502078653002202103653002302125100002002148700002002168700002002188856013502208       2011                        eng d  a1916-967100aSuitability of Tamarind and Some Selected Crop Seeds for the Survival and Development of Sitophilus Linearis (Herbst) (Coleoptera: Cucurlionidae)0 aSuitability of Tamarind and Some Selected Crop Seeds for the Sur  cJuly  a83-890 v33 aSitophilus oryzae (L.), Sitophilus zeamais (Mots.) and Sitophilus granarius (L.) are major pests of stored grain, especially on cereals. Sitophilus linearis (Herbst) a congeneric of the afore-listed species was obtained from seeds of a wild plant, Tamarindus indica L. The development of S. linearis on some selected crop seeds as well as tamarind seeds was investigated in the laboratory at 25.3-30.3 [degree]C and 75.0% [plus or minus] 5.0 RH. One hundred grammes each of heat sterilized maize, rice, sorghum, millet, cowpea and tamarind seeds were infested with 100 unsexed adults of S. linearis and observed to determine the suitability of the different substrates for the development of the weevil. Sitophilus linearis bred and multiplied on the tamarind seeds but not on the selected crop seeds in the laboratory. Mean developmental period on the tamarind seeds was 32 days. The highest number (51) of adults that emerged on the first day of emergence was from cultures set up with one-week old adult insects. The highest mean weight (3.9mg) was also recorded on the cultures set up with one-week old insects. However, both mean number of emerged adults and mean adult weight were not significantly different (P>0.05). A significant difference was recorded in the mean percentage survival period of S. linearis on the different substrates (P<0.05) with the longest survival recorded on tamarind seeds. It can therefore be concluded that Sitophilus linearis, though a member of the family of grain weevils, could not reproduce on the cereal substrates or the cowpea seeds presented to it in the laboratory. If further studies confirm these findings, then Sitophilus linearis may pose no threat to cereal production due to its inability to survive and reproduce on them.10aSitophilus granarius10aSitophilus oryzae10aSitophilus zeamais1 aAdebayo, R., A.1 aAyertey, J., N.1 aCobblah, M., A.  uhttps://weevil.myspecies.info/suitability-tamarind-and-some-selected-crop-seeds-survival-and-development-sitophilus-linearis-herbs