02077nas a2200181   4500008004100000020001400041245018300055210006900238260000900307300001200316490000800328520134500336653001701681100002301698700001801721700002101739856013501760       2010                        eng d  a0022-049300aMolecular Identification of Larval Stages of Otiorhynchus (Coleoptera: Curculionidae) Species Based on Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis0 aMolecular Identification of Larval Stages of Otiorhynchus Coleop  cJune  a898-9070 v1033 aA couple of different members of the coleopteran genus Otiorhynchus (Coleoptera: Curculionidae) are becoming increasingly important as pests of nursery and ornamental plants in global horticulture. Although adult weevils are morphologically distinguishable by skilled personnel, high potential for misidentification is given for cryptic larval stages. For developing and applying efficient pest management strategies the determination of the respective species is however a prerequisite, because each species may have a different phenology or a varying susceptibility to pesticides. Here, we report on the development of a diagnostic polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) method for differentiation among 16 Otiorhynchus and seven other weevil species independent of their developmental stage. An [approximately]780-bp fragment of the mitochondrial cytochrome oxidase subunit II was amplified and subsequently digested with at most four restriction enzymes generating species-specific fragment patterns. The assay was validated on a total of 127 individuals and the obtained fragment patterns correctly identified 23 different weevil species. The PCR-RFLP method reported here is cost-effective, robust, and fast and could be used in the future by plant protection services for diagnostic purposes.10aOtiorhynchus1 aHirsch, Jacqueline1 aSprick, Peter1 aReineke, Annette  uhttps://weevil.myspecies.info/molecular-identification-larval-stages-otiorhynchus-coleoptera-curculionidae-species-based-polymeras02256nas a2200205   4500008004500000020001400045245020300059210006900262260001100331300001400342490000800356520142400364653001401788653002601802100002401828700002001852700002101872700002201893856013501915       2008                        Engldsh    a0022-049300aMitochondrial nucleotide variability in invasive populations of the root weevil Diaprepes abbreviatus (Coleoptera: Curculionidae) of Florida and preliminary assessment of Diaprepes sp. from Dominica0 aMitochondrial nucleotide variability in invasive populations of   cAugust  a1443-14540 v1013 aDiaprepes abbreviatus (L.) (Coleoptera: Curculionidae) is a root weevil introduced into the United States from the Caribbean in 1964. It is associated with >300 plants, including citrus, sugarcane, and potatoes. D. abbreviatus is widespread in Florida, and it has recently been detected in limited areas of California and Texas. The purpose of this research is to evaluate the utility of 16S ribosomal (16S rRNA) and cytochrome oxidase I (COI) mitochondrial markers for the delineation of genetic populations of D. abbreviatus in Florida and for the characterization of patterns of dispersion among these populations. We also assessed these markers as genetic tools for the clarification of taxonomic uncertainties in specimens from Dominica (Lesser Antilles). We analyzed 111 weevils from six Florida populations and six specimens from Dominica. In Florida, we found three haplotypes with only one haplotype in each population. Florida haplotypes differed by one to three nucleotide substitutions, possibly the result of a recent divergence from one source population or three different introductions from closely related populations from the Caribbean. In contrast, specimens from Dominica showed a high genetic variability with three 16S haplotypes and six unique COI haplotypes, delineating two mitochondrial clades. We show that these mitochondrial markers are useful for phylogeographic studies of D. abbreviatus.10aDiaprepes10aDiaprepes abbreviatus1 aAscunce, Marina, S.1 aErnst, Joel, A.1 aClark, Annemarie1 aNigg, Herbert, N.  uhttps://weevil.myspecies.info/mitochondrial-nucleotide-variability-invasive-populations-root-weevil-diaprepes-abbreviatus-coleopte