01480nas a2200181   4500008004100000020001400041245011100055210006900166260000900235300001200244490000700256520081100263653002801074100001601102700002201118700002901140856012901169       2007                        eng d  a0334-511400aA new record of fungus-beetle symbiosis in Scolytodes bark beetles (Scolytinae, Curculionidae, Coleoptera)0 anew record of fungusbeetle symbiosis in Scolytodes bark beetles   c2007  a151-1590 v433 aThe most evolutionarily advanced form of symbiosis between wood-decaying fungi and wood-boring beetles (Coleoptera, Curculionidae: Scolytinae and Platypodinae) is the ambrosial habit, or fungus fanning. Here we present a discovery of a new origin of the ambrosia symbiosis in Scolytodes unipunctatus. Feeding on symbiotic fungi and the spatial organization of the gallery system of S. unipunctatus is typical for ambrosia beetles, but not for phylogenetically related phloeophagous species. S. unipunctatus is associated with the fungal genera Raffaelea, Graphium, and Gondwanamyces; the association of the latter with scolytines is documented here for the first time. The fungi were identified using morphological characters and 18S, 28S and ITS regions of rDNA. We report four undescribed fungus species.10aScolytodes unipunctatus1 aHulcr, Jiri1 aKolarik, Miroslav1 aKirkendall, Lawrence, R.  uhttps://weevil.myspecies.info/new-record-fungus-beetle-symbiosis-scolytodes-bark-beetles-scolytinae-curculionidae-coleoptera02258nas a2200229   4500008004100000020001400041245012400055210006900179260000900248300001100257490000700268520144800275653001301723100002201736700002701758700002601785700002501811700002201836700001901858700002301877856012801900       2007                        eng d  a0334-511400aComposition of the bacterial community in the gut of the pine engraver, Ips pini (Say) (Coleoptera) colonizing red pine0 aComposition of the bacterial community in the gut of the pine en  c2007  a97-1040 v433 aThe gut bacterial community of a bark beetle, the pine engraver Ips pini (Say), was characterized using culture-dependent and culture- independent methods. Bacteria from individual guts of larvae, pupae and adults were cultured and DNA was extracted from samples of pooled larval guts. Analysis of 16S rRNA gene sequences amplified directly from the gut community suggests that the gut bacterial communities associated with I. pini are relatively simple, compared to many other systems. Six bacterial genera from four classes were detected by culturing gut bacteria from larvae, pupae and adults. Two genera, Pantoea and Stenotrophomonas ([gamma]-Proteobacteria) were found in all life stages of I. pini, consistently in larvae and adults, and less commonly in pupae. Sequences that affiliate with the Enterobactereaceae of the [gamma]-Proteobacteria were found in 95% of the clones sampled. The Enterobactereaceae genera, Pantoea and Erwinia, accounted for 88% of all clone sequences. These results are consistent with previous work indicating that another bark beetle, the southern pine beetle, Dendroctonus frontalis Zimmerman, also has a relatively simple gut flora, compared with wood colonizing insects such as wood borers and termites. The composition and abundance of bacteria associated with different life stages of I. pini are possibly associated with specific functions of the gut bacterial communities of larvae, pupae, and adults10aIps pini1 aDelalibera, Italo1 aVasanthakumar, Archana1 aBurwitz, Benjamin, J.1 aSchloss, Patrick, D.1 aKlepzig, Kier, D.1 aHandelsman, Jo1 aRaffa, Kenneth, F.  uhttps://weevil.myspecies.info/composition-bacterial-community-gut-pine-engraver-ips-pini-say-coleoptera-colonizing-red-pine