01596nas a2200193   4500008004100000020001400041245014200055210006900197260001100266300001000277490000700287520088300294653002801177100001301205700001601218700001601234700001701250856013501267       2010                        eng d  a1000-538200aAssistant identification of several species of poplar borer larva based on different restriction enzyme characteristic in their COI genes0 aAssistant identification of several species of poplar borer larv  cMay 25  a83-870 v383 aDNA fragments of COI gene of larvae of Cryptorrhynchus lapathi ( Linnaeus), Saperda populnea ( Linnaeus), Xylotrechus rusticus (Linnaeus), and Anoplophora glabripennis (Motschulsky), the main poplar stem-boring pests in Heilongjiang Province, were amplified by PCR, and then sequenced and analyzed. Maps of COI gene restriction enzyme of the four species of pests were obtained. Moreover, three restriction enzymes were selected to cut the amplified COI gene fragments of X. rusticus, C. lapathi, and A. glabripennis specifically, but the COI gene of S. populnea could not be cut by any of these three restriction enzymes. Therefore, the assistant method could be applied to the inspection and quarantine of these kinds of pests, based on the primary identification of pests by their morphological characters and damage features, which would improve the identification accuracy.10aCryptorrhynchus lapathi1 aMa, Jian1 aLi, Chengde1 aDeng, Liwen1 aDu, Wensheng  uhttps://weevil.myspecies.info/assistant-identification-several-species-poplar-borer-larva-based-different-restriction-enzyme-cha-0