@article {13309,
	title = {Transcriptome and full-length cDNA resources for the mountain pine beetle, Dendroctonus ponderosae Hopkins, a major insect pest of pine forests},
	journal = {Insect Biochemistry and Molecular Biology},
	volume = {42},
	number = {8},
	year = {2012},
	month = {Aug},
	pages = {525-536},
	abstract = {Bark beetles (Coleoptera: Curculionidae: Scolytinae) are major insect pests of many woody plants around the world. The mountain pine beetle (MPB), Dendroctonus ponderosae Hopkins, is a significant historical pest of western North American pine forests. It is currently devastating pine forests in western North America - particularly in British Columbia, Canada - and is beginning to expand its host range eastward into the Canadian boreal forest, which extends to the Atlantic coast of North America. Limited genomic resources are available for this and other bark beetle pests, restricting the use of genomics-based information to help monitor, predict, and manage the spread of these insects. To overcome these limitations, we generated comprehensive transcriptome resources from fourteen full-length enriched cDNA libraries through paired-end Sanger sequencing of 100,000 cDNA clones, and single-end Roche 454 pyrosequencing of three of these cDNA libraries. Hybrid de novo assembly of the 3.4 million sequences resulted in 20,571 isotigs in 14,410 isogroups and 246,848 singletons. In addition, over 2300 non-redundant full-length cDNA clones putatively containing complete open reading frames, including 47 cytochrome P450s, were sequenced fully to high quality. This first large-scale genomics resource for bark beetles provides the relevant sequence information for gene discovery; functional and population genomics; comparative analyses; and for future efforts to annotate the MPB genome. These resources permit the study of this beetle at the molecular level and will inform research in other Dendroctonus spp. and more generally in the Curculionidae and other Coleoptera. (C) 2012 Elsevier Ltd. All rights reserved.},
	keywords = {Scolytinae},
	isbn = {0965-1748},
	author = {Keeling, Christopher I. and Henderson, Hannah and Li, Maria and Yuen, Mack and Clark, Erin L. and Fraser, Jordie D. and Huber, Dezene P. W. and Liao, Nancy Y. and Docking, T. Roderick and Birol, Inanc and Chan, Simon K. and Taylor, Greg A. and Palmquist, Diana and Jones, Steven J. M. and Bohlmann, Joerg}
}
@article {12583,
	title = {Successful colonization, reproduction, and new generation emergence in live interior hybrid spruce Picea engelmannii x glauca by mountain pine beetle Dendroctonus ponderosae},
	journal = {Agricultural and Forest Entomology},
	volume = {11},
	number = {1},
	year = {2009},
	month = {February},
	pages = {83-89},
	abstract = {1 Although mountain pine beetle Dendroctonus ponderosae Hopkins are able to utilize most available Pinus spp. as hosts, successful colonization and reproduction in other hosts within the Pinaceae is rare.2 We observed successful reproduction of mountain pine beetle and emergence of new generation adults from interior hybrid spruce Picea engelmannii x glauca and compared a number of parameters related to colonization and reproductive success in spruce with nearby lodgepole pine Pinus contorta infested by mountain pine beetle.3 The results obtained indicate that reduced competition in spruce allowed mountain pine beetle parents that survived the colonization process to produce more offspring per pair than in more heavily-infested nearby pine.4 We also conducted an experiment in which 20 spruce and 20 lodgepole pines were baited with the aggregation pheromone of mountain pine beetle. Nineteen pines (95\%) and eight spruce (40\%) were attacked by mountain pine beetle, with eight (40\%) and three (15\%) mass-attacked, respectively.5 Successful attacks on nonhost trees during extreme epidemics may be one mechanism by which host shifts and subsequent speciation events have occurred in Dendroctonus spp. bark beetles.},
	keywords = {Dendroctonus ponderosae},
	isbn = {1461-9555},
	author = {Huber, Dezene P. W. and Aukema, Brian H. and Hodgkinson, Robert S. and B.S. Lindgren}
}
@article {12584,
	title = {Expression patterns of bark beetle cytochromes p450 during host colonization: likely physiological functions and potential targets for pest management},
	journal = {U S Forest Service Rocky Mountain Research Station Proceedings RMRS-P},
	volume = {45},
	year = {2007},
	month = {June},
	pages = {41-42},
	author = {Huber, Dezene P. W. and Erickson, Melissa and Leutenegger, Christian and Bohlmann, Joerg and Seybold, Steven J.}
}
@article {10974,
	title = {Responses of Dendroctonus brevicomis (Coleoptera: Curculionidae) in Behavioral Assays: Implications to Development of a Semiochemical-Based Tool for Tree Protection},
	journal = {Journal of Economic Entomology},
	volume = {105},
	number = {1},
	year = {2012},
	month = {Feb},
	pages = {149-160},
	abstract = {Currently, techniques for managing western pine beetle, Dendroctonus brevicomis LeConte (Coleoptera: Curculionidae, Scolytinae), infestations are limited to tree removals (thinning) that reduce stand density and presumably host susceptibility, and/or the use of insecticides to protect individual trees. There continues to be significant interest in developing an effective semiochemical-based tool for protecting trees from D. brevicomis attack, largely as an alternative to conventional insecticides. The responses of D. brevicomis to tree volatiles and verbenone were documented in eight experiments (trapping assays) conducted over a 4-yr period in which 88,942 individuals were collected. Geraniol, a tree volatile unique to Pinus ponderosa that elicits female-specific antennal responses in D. brevicomis, did not affect D. brevicomis behavior. Blends of two green leaf alcohols (hexanol + (Z)-3-hexen-1-ol) tested at two release rates (5.0 and 100.0 mg/d) had no effect on the response of D. brevicomis to attractant-baited traps. A nine-component blend ( benzaldehyde, benzyl alcohol, guaiacol, nonanal, salicylaldehyde, (E)-2-hexenal, (E)-2-hexen-1-ol, (Z)-2-hexen-1-ol, and (-)-verbenone; NAVV) and subsequent revisions of this blend disrupted the response of D. brevicomis to attractant-baited traps in all experiments. The inhibitory effect of a revised five-component blend (nonanal, (E)-2-hexenal, (E)-2-hexen-1-ol, (Z)-2-hexen-1-ol, and (-)-verbenone; NAVV5) on the response of mountain pine beetle, D. ponderosae Hopkins, to attractant-baited traps was also documented. Acetophenone significantly reduced D. brevicomis attraction, but was not as effective as verbenone alone. Acetophenone increased the effectiveness of NAVV5 in one of two experiments. Furthermore, by adding acetophenone to NAVV5 we were able to remove the aldehydes from NAVV5 without compromising effectiveness, resulting in a novel four-component blend ( acetophenone, (E)-2-hexen-1-ol + (Z)-2-hexen-1-ol, and (-)-verbenone; Verbenone Plus). We discuss the implications of these and other results to development of Verbenone Plus as a semiochemical-based tool for management of D. brevicomis and D. ponderosae infestations.},
	keywords = {Dendroctonus brevicomis},
	isbn = {0022-0493},
	author = {Fettig, Christopher J. and McKelvey, Stephen R. and Dabney, Christopher P. and Huber, Dezene P. W.}
}
@article {9973,
	title = {Differences in the constitutive terpene profile of lodgepole pine across a geographical range in British Columbia, and correlation with historical attack by mountain pine beetle},
	journal = {Canadian Entomologist},
	volume = {142},
	number = {6},
	year = {2010},
	month = {Nov},
	pages = {557-573},
	abstract = {The mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae), is a destructive insect pest in western Nearctic conifer forests. Currently, British Columbia, Canada, is experiencing the largest recorded outbreak of this insect, including areas that historically have had low climatic suitability for it. We analyzed 26 constitutive resin terpenes in phloem samples from British Columbia lodgepole pine (Pinus contorta) populations to test for differential resistance to mountain pine beetle attack, based upon the likelihood of previous exposure to mountain pine beetle. We assessed sampled trees for number of mountain pine beetle attacks, number of pupal chambers, and tree survival the following spring. Significant differences were found when levels of certain terpenes in lodgepole pine populations that had likely experienced substantial mountain pine beetle infestations in the past were compared with those in populations that likely had not experienced large outbreaks of mountain pine beetle. Although we expected southern pine populations to contain more total terpenes than northern populations, owing to higher historical exposure to the beetle, the converse was found. Northern populations generally had higher levels of constitutive terpenes and beetle attack than southern populations. Because several terpenes are kairomones to the mountain pine beetle and also serve as precursors for the synthesis of pheromones, the lower levels of terpenes expressed by lodgepole pines from the historical range of the mountain pine beetle may render them less chemically perceptible to foraging beetles.},
	keywords = {Dendroctonus ponderosae},
	isbn = {0008-347X},
	author = {Clark, Erin L. and Carroll, Allan L. and Huber, Dezene P. W.}
}
