@article {12589, title = {Protein profiles of Gonipterus scutellatus (Coleoptera: Curculionidae) development stages}, journal = {Revista Colombiana de Entomologia}, volume = {33}, number = {2}, year = {2007}, month = {July-December}, pages = {98-103}, abstract = {In 1998 the eucalyptus weevil, Gonipterus scutellatus (Coleoptera: Curculionidae), a major defoliator of the genus Eucalyptus, which causes growth and economic losses, was detected in Chile. This has caused concern because more than 525,000 ha are planted with species of eucalypt. To understand the protein responses of development stages of G. scutellatus fed on the three species of eucalypt trees most important in the country, electrophoretic profiles were obtained and characterized on polyacrylamide gels under denaturizing conditions, and marker proteins were sought through gel densitometry. Proteins became more numerous with insect development. Protein bands were concentrated between 20 and 120 kDa, with the exception of eggs, which presented lower values. The appearance of distinct bands of proteins in the extracts from larvae and adults of G. scutellatus fed on different species of eucalypts reflected changes in their metabolism. These results are useful for integrated pest management, as the identification of marker proteins is possibly associated with metabolic patterns related to the consumption of host plants. Changes in insect diet may affect the marker proteins, indicating a differential ability of G. scutellatus to use host plants.}, keywords = {Gonipterus scutellatus}, isbn = {0120-0488}, author = {Huerta F, Amanda and Chiffelle G, Italo and Serrano G, Maryi and Vasquez T, Tatiana} } @article {12590, title = {Susceptibility of eucalyptus species to Gonipterus scutellatus and electrophoretic profiles of adult marker proteins}, journal = {Agrociencia (Montecillo)}, volume = {42}, number = {3}, year = {2008}, month = {1 de Abril-15 de}, pages = {327-334}, type = {Article}, abstract = {In Chile, the Australian weevil Gonipterus scutellatus Gyllenhal (Coleoptera: Curculionidae), specific to eucalyptus, was first detected in 1998. It has spread through the IV to IX Regions and could affect more than 525 thousand ha of Eucalyptus. The insect feeds on new foliage and causes losses in growth. The objectives of this study were to evaluate the susceptibility of Chile{\textquoteright}s most important eucalyptus species to damage by G. scutellatus, to characterize the molecular weight (kDa) of proteins of adult weevils that feed on the trees, and to identify marker proteins of the insects associated with the species of eucalyptus on which they feed. Susceptibility of Eucalyptus camaldulensis Dehnh., E. globulus sp. globulus Labill. and E. robusta Smith to adult insects was measured by leaf area loss. The proteins of the adults were analyzed by electrophoresis, comparing size and intensity of the bands of gels. E. camaldulensis was more susceptible (p<=0.05; 12.93\% leaf area loss) than E. robusta (6.36\%) or E. globulus (5.46\%). Adults fed with E. robusta had the highest number of proteins (22). Those fed with each one of the three eucalyptus species separately had 15 proteins in common, and nine exhibited variations (marker proteins). Adult weevils fed E. robusta had seven marker proteins; E. camaldulensis and E. globulus had three marker proteins each. Weevils fed E. robusta had three marker proteins exclusive to these insects (9, 31 and 38 kDa), while those fed with E. camaldulensis and E. globulus had only one each (35 and 47 kDa). Thus, the three groups of weevils fed with different eucalyptus species had different protein profiles.}, keywords = {Gonipterus scutellatus}, isbn = {1405-3195}, author = {Huertas-Fuentes, Amanda and Chiffelle-Gomez, Italo and Serrano-Garzon, Maryi and Vazquez-Silva, Tatiana and Araya-Clericus, Jaime} } @article {8493, title = {Gonipterus scutellatus s.l. in Montecristo Island (Tuscany): should its first introduction to the Palearctic region be backdated to the 19th century? (Coleoptera Curculionidae)}, journal = {Bollettino della Societa Entomologica Italiana}, volume = {144}, number = {1}, year = {2012}, month = {gennaio-aprile}, pages = {19-22}, abstract = {A population of Gonipterus scutellatus sensu lato (Coleoptera Curculionidae), a pest of Eucalyptus spp., was found on February 2011, for the first time in Tuscany, in the Integral Natural Reserve of Montecristo Island (Central Italy) as the possible result of a late 19th-century introduction on eucalyptus trees from Australia.}, keywords = {Gonipterus scutellatus}, isbn = {0373-3491}, author = {Mazza, Giuseppe and Abbazzi, Piero and Andriolo, Luciana and Inghilesi, Alberto Francesco and Marziaj, Giorgio and Sposimo, Paolo and Zoccola, Antonio and Gherardi, Francesca} }