02363nas a2200253   4500008004100000020001400041245011500055210006900170260000800239300001400247490000700261520148400268653002601752653002801778100002601806700001801832700002201850700001901872700002001891700002501911700001801936700002001954856013501974       2011                        eng d  a0962-108300aGene genealogies reveal cryptic species and host preferences for the pine fungal pathogen Grosmannia clavigera0 aGene genealogies reveal cryptic species and host preferences for  cJun  a2581-26020 v203 aGrosmannia clavigera is a fungal pathogen of pine forests in western North America and a symbiotic associate of two sister bark beetles: Dendroctonus ponderosae and D. jeffreyi. This fungus and its beetle associate D. ponderosae are expanding in large epidemics in western North America. Using the fungal genome sequence and gene annotations, we assessed whether fungal isolates from the two beetles inhabiting different species of pine in epidemic regions of western Canada and the USA, as well as in localized populations outside of the current epidemic, represent different genetic lineages. We characterized nucleotide variations in 67 genomic regions and selected 15 for the phylogenetic analysis. Using concordance of gene genealogies and distinct ecological characteristics, we identified two sibling phylogenetic species: Gc and Gs. Where the closely related Pinus ponderosa and P. jeffreyi are infested by localized populations of their respective beetles, Gc is present. In contrast, Gs is an exclusive associate of D. ponderosae mainly present on its primary host-tree P. contorta; however, in the current epidemic areas, it is also found in other pine species. These results suggest that the host-tree species and the beetle population dynamics may be important factors associated with the genetic divergence and diversity of fungal partners in the beetle-tree ecosystems. Gc represents the original G. clavigera holotype, and Gs should be described as a new species.10aDendroctonus jeffreyi10aDendroctonus ponderosae1 aAlamouti, Sepideh, M.1 aWang, Vincent1 aDiGuistini, Scott1 aSix, Diana, L.1 aBohlmann, Joerg1 aHamelin, Richard, C.1 aFeau, Nicolas1 aBreuil, Colette  uhttps://weevil.myspecies.info/gene-genealogies-reveal-cryptic-species-and-host-preferences-pine-fungal-pathogen-grosmannia-clavige