02260nas a2200169   4500008004100000020001400041245012600055210006900181260000800250300001200258490000700270520161100277100002201888700002301910700002201933856013501955       2010                        eng d  a0046-225X00aNitrogen Concentration in Mountain Pine Beetle Larvae Reflects Nitrogen Status of the Tree Host and Two Fungal Associates0 aNitrogen Concentration in Mountain Pine Beetle Larvae Reflects N  cJun  a821-8260 v393 aIndividual lodgepole pines (Pious conform) were fertilized with urea at nitrogen (N) inputs equivalent to 0, 315, or 630 kg/ha Four months after application of the fertilizer, inner bark tissue N concentrations were significantly higher in the trees that had received the low dose (315 kg/ha) fertilization treatment than in the control trees; trees that had received the high-dose treatment (630 kg/ha) were intermediate and not significantly different from either of the other treatments. There was a significant positive correlation between N concentration in inner bark tissue and larval mountain pine beetle, Dendroctonus ponderosae (Coleoptera Curculionidae, Scolytinae). In vitro studies on synthetic growth media examined effects of temperature and N concentration on N concentration of two common fungal associates of the mountain pine beetle (Ophiostoma clavigerum and Ophiostoma montium). Increasing N concentration in growth media significantly increased fungal N concentrations in both O. clavigerum and O. montium. Furthermore, N concentration was consistently higher in O. clavigerum than in O. montium. Neither species had sufficient growth at 30[degree]C, nor did O. clavigerum at 15[degree]C, to test N concentration. However, for O. montium, increasing temperatures decreased fungal N concentrations. There was no correlation between N concentration of O. clavigerum and growth temperature. Potential impacts of ingestion of the fungal species by developing mountain pine beetle larvae-infesting trees under various environmental conditions such as increasing temperatures are discussed.1 aCook, Stephen, P.1 aShirley, Brian, M.1 aZambino, Paul, J.  uhttps://weevil.myspecies.info/nitrogen-concentration-mountain-pine-beetle-larvae-reflects-nitrogen-status-tree-host-and-two-fungal