Assistant identification of several species of poplar borer larva based on different restriction enzyme characteristic in their COI genes

DNA fragments of COI gene of larvae of Cryptorrhynchus lapathi ( Linnaeus), Saperda populnea ( Linnaeus), Xylotrechus rusticus (Linnaeus), and Anoplophora glabripennis (Motschulsky), the main poplar stem-boring pests in Heilongjiang Province, were amplified by PCR, and then sequenced and analyzed. Maps of COI gene restriction enzyme of the four species of pests were obtained. Moreover, three restriction enzymes were selected to cut the amplified COI gene fragments of X. rusticus, C. lapathi, and A. glabripennis specifically, but the COI gene of S. populnea could not be cut by any of these three restriction enzymes. Therefore, the assistant method could be applied to the inspection and quarantine of these kinds of pests, based on the primary identification of pests by their morphological characters and damage features, which would improve the identification accuracy.